Kravchun PG, Leontieva FS, Ashukina NO, Danyshchuk ZM, Marushchak OP, Dielievska VYu
Aim: To analyze the influence of heat stable anti-A and anti-B antibodies of the persons with anti-A and anti-B absorbing ability of erythrocytes different from their blood group type on erythrocyte membrane.
Materials and methods: Reaction of hemagglutination with complement at room temperature, 4°C, 37°C with the heated sera, elution and method of absorption were used.
Results: The heated sera of the persons with anti-A and anti-B absorbing abilities of erythrocytes corresponding to their blood group characteristics increased the size of the erythrocytes during incubation with complement at room temperature on the contrary to the heated sera of the persons with absorbing ability of erythrocytes different from their blood group type. The contact of the studied erythrocytes with antihelmintic drug fenbendazole decreased the level of anti-A antibodies obtained by elution after the absorbtion of the person’s erythrocytes with anti-A serum. The person’s serum showed high level of antibodies against Ascaris lumbricoides (1.25 s/co as compared to the normal <1.0 s/co) and Toxocara canis (1.8 s/co, 1:200 as compared to the normal <1.0 s/co).
Conclusion: The heated sera of the persons with different from their blood group type anti-A or anti-B absorbing ability of erythrocytes did not increase the size of erythrocytes so much as compared to the sera of the persons with absorbing ability of erythrocytes corresponding to their blood group type. The decrease of anti-A antibodies in hemagglutination reaction and after elution might be associated with the involvement of hemagglutinins due to Ascaris lumbricoides infection.